DNA

Part:BBa_K4687022

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


Donor:crtEb

CRISPR-MAD7 nuclease binds to the downstream of the PAM region of the target DNA under the guidance of sgRNA. The active region of the protein will cut out a sticky end of the target DNA, and then introduce a DNA fragment homologous to the end (donor DNA) and the broken DNA for homologous recombination repair. In this project, we designed donor DNA to interact with CRISPR-MAD7 gene editing systems to achieve targeted gene knockout.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1148
    Illegal PstI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1148
    Illegal NheI site found at 265
    Illegal PstI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1148
    Illegal PstI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1148
    Illegal PstI site found at 652
    Illegal NgoMIV site found at 1563
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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